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Dysregulation of RNA-binding proteins in rheumatoid arthritis and osteoarthritis
Announcing a new publication for Acta Materia Medica magazine. Single-cell RNA sequencing (SCRNA-Seq) data from published datasets were obtained to examine the expression and dysregulation of RNA-binding proteins (RBPS), which are critical for alternative mRNA splicing and translational control in rheumatoid arthritis (RA). How RBP regulation differs between RA and osteoarthritis (OA) was examined using RBP for cell subclustering. Quantitative polymerase chain reactions (PCRs) were performed to confirm differentially expressed RBPs in RA fibroblastic synoviocytes (FLSS) and OA-FLS, as well as in collagen-induced arthritis (CIA) mice and control mice. Additionally, the bulk RNA-seq data were collected and RBP alternative splicing events (ASE) co-expression analyzes were performed to...
Dysregulation of RNA-binding proteins in rheumatoid arthritis and osteoarthritis
Announcing a new release forActa Materia MedicaMagazine. Single-cell RNA sequencing (SCRNA-Seq) data from published datasets were obtained to examine the expression and dysregulation of RNA-binding proteins (RBPS), which are critical for alternative mRNA splicing and translational control in rheumatoid arthritis (RA). How RBP regulation differs between RA and osteoarthritis (OA) was examined using RBP for cell subclustering.
Quantitative polymerase chain reactions (PCRs) were performed to confirm differentially expressed RBPs in RA fibroblastic synoviocytes (FLSS) and OA-FLS, as well as in collagen-induced arthritis (CIA) mice and control mice. Additionally, the bulk RNA-seq data were collected and RBP alternative splicing events (ASE) co-expression analyzes were performed to reveal the potential regulatory role of RA-related RBPs with ASD. Significant variations in the relative proportions of cell subtypes were demonstrated between RA and OA with RBPs downregulated, RBPs overregulated in each cell type, and showing high specificity for certain subsets. One hundred five upregulated and 133 downregulated RBPs were identified in fibroblasts. Y-box binding protein 3 (YBX3) and splicing factor 3B subunit 6 (SF3B6) were found to be upregulated in RA-FLS and CIA mice) were downregulated in RA-FLS. The RA group showed stronger interactions with cell types compared to the OA group with improved signaling pathways such as: B. Fibronectin 1 cluster of differentiation 44 (FN1-CD44) and CXC motif chemokine ligand 12-CXC motif chemokine receptor 4 (CXCL12-CXCR4).
In addition, three upregulated genes (spectrin repeat containing nuclear envelope protein 2, containing protein 2 [SYNE2]S100 calcium binding protein A9 [S100A9] and interferon -induced protein containing tetratricopeptide repeats 3 [IFIT3]) and four downregulated genes (ribonuclease 1 [RNASE1]granulin [GRN]Fn1 and sorbin and SH3 domain containing 2 [SORBS2]) were co-expressed in RA-associated RBPs and ASES.
These results suggest that dysregulation of RBPs may contribute to the development of RA and provide potential targets for therapeutic interventions.
Sources:
Luo, H.,et al. (2025). Deciphering the regulatory programs of RNA binding proteins in rheumatoid arthritis through single-cell transcriptome analysis. Acta Materia Medica. doi.org/10.15212/amm-2024-0034.